Dr Marcus Droege

Title: Re-sequencing of the human genome using the 454 Genome Sequencer systems FLX & Junior

The Genome Sequencer FLX Systems (GS FLX and GS Junior), powered by 454 Sequencing, are based on a next-generation DNA sequencing technology featuring a unique mix of long reads, exceptional data accuracy and shortest run time. It has been proven to be the most versatile of all currently available next-generation sequencing technologies, supporting more than 700 high profile studies in over 7 applications categories. 454 technology users have pursued innovative research in de novo sequencing, re-sequencing of whole genomes and target DNA regions, metagenomics, and RNA analysis. 454 Sequencing is a powerful tool for human genetics research, including re-sequencing the complete human exome or detection of low frequency somatic mutations linked to cancer.

This presentation will provide a short overview about the 454 Sequencing technology, and will focus on applications possible to address with the Genome Sequencer systems, with special emphasis on human re-sequencing (data presentation). It will also provide information on the newest product developments, including the 2010 launch of kits allowing to generate read lengths of >700 - 1000 or the first next generation benchtop instrument, the GS Junior.